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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in many tumors, which results in chromosome instability, and therefore may perhaps contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two additional splice variants ended up noted but detailed nucleotide sequence data isn't nonetheless offered.
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Blocking peptides are peptides that bind especially to the goal antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.
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This information outlines even more feasible optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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